Application
Western blot of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibod| Product Name | GABAB Receptor (phospho Ser923) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GABA-B receptor 1 |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Phosphorylation enhances GABAB receptor effector coupling (Couve et al., 2004). Phosphorylation of Ser923 is thought to be important in the regulation of GABAB receptor function.1) Couve A, Calver AR, Fairfax B, Moss SJ, Pangalos MN (2004) Unravelling the unusual signaling proerties of the GABA(B) receptor. Biochem. Pharmacol. Oct 12;68(8):1527-36. |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser923 of GABAB R1. |
| Modification | p-Ser923 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | BovineHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Gabbr1 |
|---|---|
| Alternative Names | GABA-B receptor 1 |
| Molecular Weight(MW) | 102 |
Application
Western blot of rat synaptic membrane showing specific immunolabeling of the ~102k GABAB R1 protein phosphorylated at Ser923 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser923 GABAB antibod| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GABAB Receptor antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
