GA Binding Protein . chain (GABP-. subunit, GABPA, nuclear respiratory factor-2 subunit . transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.1) Xue, H.H., et al., (2004) Nat.Immunol.5(10), 1036-1044.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GABPA.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
GABPA antibody can be used for detection of GABPA by ELISA at 1:312500. GABPA antibody can be used for detection of GABPA by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
GABPA antibody can be used for detection of GABPA by ELISA at 1:312500. GABPA antibody can be used for detection of GABPA by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GABPA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL.