Application
GABRA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of GABRA4 Antibody in CEM cell line lysates (35ug/lane)| Product Name | GABRA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4, GABRA4 |
| Product description | GABRA4 is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor.1) Guilmatre,A., et.al., Arch. Gen. Psychiatry 66 (9), 947-956 (2009) |
| Immunogen | This GABRA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 350-377 amino acids from the Central region of human GABRA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GABRA4 |
|---|---|
| Alternative Names | Gamma-aminobutyric acid receptor subunit alpha-4 GABA(A) receptor subunit alpha-4 GABRA4 |
| Molecular Weight(MW) | 62 kDa |
Application
GABRA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of GABRA4 Antibody in CEM cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
