Application
Western blot analysis of lysate from 293 cell line, using GADD45GIP1 Antibody at 1:1000.Application
Flow cytometric analysis of MCF-7 cells using GADD45GIP1 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Product Name | GADD45GIP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Growth arrest and DNA damage-inducible proteins-interacting protein 1, 39S ribosomal protein L59, mitochondrial, MRP-L59, CKII beta-associating protein, CR6-interacting factor 1, CRIF1, Papillomavirus L2-interacting nuclear protein 1, PLINP, PLINP-1, p53-r |
| Product description | Acts as a negative regulator of G1 to S cell cycle phase progression by inhibiting cyclin-dependent kinases. Inhibitory effects are additive with GADD45 proteins but occurs also in the absence of GADD45 proteins. Acts as a repressor of the orphan nuclear receptor NR4A1 by inhibiting AB domain-mediated transcriptional activity. May be involved in the hormone-mediated regulation of NR4A1 transcriptional activity. May play a role in mitochondrial protein synthesis.1) Goernemann J.,et al.Virology 303:69-78(2002). |
| Immunogen | This GADD45GIP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 13-45 amino acids from the N-terminal region of human GADD45GIP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GADD45GIP1 |
|---|---|
| Alternative Names | Growth arrest and DNA damage-inducible proteins-interacting protein 1 39S ribosomal protein L59 mitochondrial MRP-L59 CKII beta-associating protein CR6-interacting factor 1 CRIF1 Papillomavirus L2-interacting nuclear protein 1 PLINP PLINP-1 p53-r |
| Molecular Weight(MW) | 25 kDa |
Application
Western blot analysis of lysate from 293 cell line, using GADD45GIP1 Antibody at 1:1000.
Application
Flow cytometric analysis of MCF-7 cells using GADD45GIP1 Antibody (N-term)(green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
