GALNAC4S-6ST is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc (4,6-SO (4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc (4SO4)-GlcA (2SO4)-GalNAc (6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. GALNAC4S-6ST may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo.1) Sawada, T., (2005) Carbohydr. Res. 340 (12), 1983-1996.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNAC4S-6ST.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GALNAC4S-6ST antibody can be used for detection of GALNAC4S-6ST by ELISA at 1:12500. GALNAC4S-6ST antibody can be used for detection of GALNAC4S-6ST by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Positive Control
1) Cat. No. 1201 - HeLa Cell Lysate
Application Notes
GALNAC4S-6ST antibody can be used for detection of GALNAC4S-6ST by ELISA at 1:12500. GALNAC4S-6ST antibody can be used for detection of GALNAC4S-6ST by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GALNAC4S-6ST antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.