Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | GALNT10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GALNT10, DKFZp586H0623, FLJ00205, FLJ11715, GalNAcT10, pp-GalNAc-T10, GALNACT10, PPGALNACT10, PPGANTASE10 |
| Product description | GALNT10 belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates.This gene belongs to the polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Following expression in insect cells, recombinant GalNAc transferase 10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both nonglycosylated and glycosylated peptide substrates. Two transcript variants encoding distinct isoforms have been identified for this gene.1) Cheng, L., (2002) FEBS Lett. 531 (2), 115-121. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNT10. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GALNT10 antibody can be used for detection of GALNT10 by ELISA at 1:62500. GALNT10 antibody can be used for detection of GALNT10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GALNT10 |
|---|---|
| Alternative Names | GALNT10 DKFZp586H0623 FLJ00205 FLJ11715 GalNAcT10 pp-GalNAc-T10 GALNACT10 PPGALNACT10 PPGANTASE10 |
| Molecular Weight(MW) | 31 kDa |
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | GALNT10 antibody can be used for detection of GALNT10 by ELISA at 1:62500. GALNT10 antibody can be used for detection of GALNT10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GALNT10 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
