Application
Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.Application
Immunofluorescence of GALNT10 in human brain tissue with GALNT10 antibody at 20 μg/mL.Application
Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | GALNT10 Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | GALNT10 Antibody: Protein glycosylation is an important biological process that is carried out by a large family of glycosyltransferases that catalyze the synthesis of oligosaccharides and glycoconjugates. Polypeptide GalNAc transferases initiate the synthesis of mucin-type oligosaccharides by transferring GalNAc from UDP-GalNAc to the hydroxyl group of either a serine or threonine residue on the polypeptide acceptor. Polypeptide galactoaminyltransferase 10 (GALNT10) belongs to the polypeptide N-acetylgalactosaminyl-transferase (pp-GalNAc-T) protein family. Following expression in insect cells, recombinant GALNT10 showed significant GalNAcT activity toward mucin-derived peptides, and it utilized both non-glycosylated and glycosylated peptide substrates. GALNT10 mRNA is highly expressed in several distinct hypothalamic, thalamic, and amygdaloid nuclei in mouse brain. At least four isoforms of GALNT10 are known to exist.1) Amado M, Almeida R, Schwientek T, et al. Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim. Biophys. Acta1999; 1473:35-53. |
| Immunogen | GALNT10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human GALNT10. The immunogen is located within amino acids 80 - 130 of GALNT10. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,IHC-P,WB |
| GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GALNT10 |
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| Alternative Names | GALNT10 Antibody: GALNACT10 PPGALNACT10 PPGANTASE10 Polypeptide N-acetylgalactosaminyltransferase 10 Polypeptide GalNAc transferase 10 GalNAc-T10 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of GALNT10 in human brain tissue with GALNT10 antibody at 2.5 μg/mL.
Application
Immunofluorescence of GALNT10 in human brain tissue with GALNT10 antibody at 20 μg/mL.
Application
Western blot analysis of GALNT10 in rat brain tissue lysate with GALNT10 antibody at (A) 1 and (B) 2 μg/mL.| Application Notes | GALNT10 antibody can be used for detection of GALNT10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | GALNT10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GALNT10 Antibody is supplied in PBS containing 0.02% sodium azide. |
