GALNT14 (EC 2.4.1.41) belongs to a large subfamily of glycosyltransferases residing in the Golgi apparatus. GALNT enzymes catalyze the first step in the O-glycosylation of mammalian proteins by transferring N-acetyl-D-galactosamine (GalNAc) to peptide substrates. GALNT14 (EC 2.4.1.41) belongs to a large subfamily of glycosyltransferases residing in the Golgi apparatus. GALNT enzymes catalyze the first step in the O-glycosylation of mammalian proteins by transferring N-acetyl-D-galactosamine (GalNAc) to peptide substrates.1) Wu, C., (2007) Biochem. Biophys. Res. Commun. 357 (2), 360-365.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNT14.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GALNT14 antibody can be used for detection of GALNT14 by ELISA at 1:1562500. GALNT14 antibody can be used for detection of GALNT14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.
Positive Control
1) OVCAR-3 Cell Lysate
Application Notes
GALNT14 antibody can be used for detection of GALNT14 by ELISA at 1:1562500. GALNT14 antibody can be used for detection of GALNT14 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GALNT14 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.