Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Product Name | GALNT4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GALNT4 catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It has a highest activity toward Muc7, EA2 and Muc2, with a lowest activity than GALNT2. This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon.1) Lampasona, V., (er) J. Thromb. Thrombolysis (2008) In press. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GALNT4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISA,WB |
| GALNT4 antibody can be used for detection of GALNT4 by ELISA at 1:12500. GALNT4 antibody can be used for detection of GALNT4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GALNT4 |
|---|---|
| Alternative Names | GALNT4 GALNAC-T4 GalNAcT4 GALNACT4 |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Application Notes | GALNT4 antibody can be used for detection of GALNT4 by ELISA at 1:12500. GALNT4 antibody can be used for detection of GALNT4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
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| Form | Liquid |
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| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GALNT4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
