Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | GAR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H/ACA ribonucleoprotein complex subunit 1, Nucleolar protein family A member 1, snoRNP protein GAR1, GAR1, NOLA1 |
| Product description | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.1) Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010) |
| Immunogen | This GAR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-114 amino acids from the Central region of human GAR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAR1 |
|---|---|
| Alternative Names | H/ACA ribonucleoprotein complex subunit 1 Nucleolar protein family A member 1 snoRNP protein GAR1 GAR1 NOLA1 |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Zebrafish |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
