Application
Western blot analysis of GAS in EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with GAS antibody at 1μg/mL.| Product Name | GAS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAS Antibody: GAS, PA1, C16orf53, PAXIP1-associated glutamate-rich protein 1, PAXIP1-associated protein 1 |
| Product description | GAS Antibody: Steroid receptor co-activators (SRCs) were initially described as nuclear receptor transcription co-activators, but they have recently been determined to co-regulate transcription initiated by other transcription factors. GAS is a recently identified glutamate-rich protein that interacts with SRC1, but not GRIP1 or AIB1, the other two members of the SRC family. GAS can also interact with the alpha subunit of the estrogen receptor (ERalpha), but not other receptors such as the retinoic acid receptor a, suggesting the interaction between GAS and ERalpha is relatively specific. Depletion of GAS by RNA interference in MCF7 cells led to a decrease in the mRNA and protein levels of ER target genes such as pS2, c-Myc and cyclin D1, indicating the role of GAS in the regulation of ER target genes. GAS has also been found to associate with an SET1-like methyltransferase complex specific for H3K4 methylation, suggesting that GAS has multiple roles in transcriptional regulation.1) Xu J, Wu RC, and O’Malley BW. Normal and cancer-related functions of the p160 steroid receptor co-activator (SCR) family. Nat. Rev. Cancer2009; 9:615-30. |
| Immunogen | GAS antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human GAS. The immunogen is located within the last 50 amino acids of GAS. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GAS antibody can be used for detection of GAS by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C16orf53 |
|---|---|
| Alternative Names | GAS Antibody: GAS PA1 C16orf53 PAXIP1-associated glutamate-rich protein 1 PAXIP1-associated protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (93%) |
Application
Western blot analysis of GAS in EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide with GAS antibody at 1μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | GAS antibody can be used for detection of GAS by Western blot at 1 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | GAS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GAS Antibody is supplied in PBS containing 0.02% sodium azide. |
