Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-3 protein.| Product Name | GAT-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gat3, Gabt4, Gabt3, Gat-3, Gat-b, Solute carrier family 6 member 11, GAT-3 |
| Product description | Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABAA receptor (GABAA-R) subtype. GABA plasma membrane transporters (GATs) influence synaptic neurotransmission by high-affinity uptake and release of GABA. To date, four distinct GABA transporters have been identified: GAT-1, GAT-2, GAT-3, and BGT-1. GAT-3 has been found to be localized to astrocytes within the cerebral cortex indicating that this transporter mediates GABA uptake into glial cells (Minelli et al., 1996).1) Minelli A, DeBiasi S, Brecha NC, Zuccarello LV, Conti F (1996) GAT-3, a high-affinity GABA plasma membrane transporter, is localized to astrocytic processes, and is not confined to the vicinity of GABAergic synapses in the cerebral cortex. J. Neurosci. 16(19):6255-64. |
| Immunogen | Peptide corresponding to amino acid residues from the C-terminal region of rat GAT-3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Slc6a11 |
|---|---|
| Alternative Names | Gat3 Gabt4 Gabt3 Gat-3 Gat-b Solute carrier family 6 member 11 GAT-3 |
| Molecular Weight(MW) | 67 |
Application
Western blot of rat hippocampal homogenate showing specific immunolabeling of the~ 67k GAT-3 protein.| Application Notes | The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | GAT-3 antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year. |
| Storage Buffer | 100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol. |
