Application
Western blot analysis of lysed extracts from 3T3 cells using GATA1 (Ab-142).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Ab-142). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (Ab-142).| Product Name | GATA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GF1, GF-1, NFE1, XLTT, ERYF1, NF-E1, XLANP, XLTDA, GATA-1, PLC1, GAT1, GATA1 |
| Product description | GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.1) Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. Trainor C D, et al. (1990) Nature. 343: 92-96. Nichols K E, et al. (2000) Nat Genet. 24: 266-270. Freson K, et al. (2001) Blood. 98: 85-92. Mehaffey M G, et al. (2001) Blood. 98: 2681-2688. |
| Immunogen | GATA1 (Ab-142) antibody was raised against a peptide sequence around aa.140~144 (R-L-S-P-D) derived from Human GATA1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA1 |
|---|---|
| Alternative Names | GF1 GF-1 NFE1 XLTT ERYF1 NF-E1 XLANP XLTDA GATA-1 PLC1 GAT1 GATA1 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of total GATA1 protein. |
| Entrez Gene | 2623 |
|---|---|
| Protein Accession | NP_002040.1 |
Application
Western blot analysis of lysed extracts from 3T3 cells using GATA1 (Ab-142).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Ab-142). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (Ab-142).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
