Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in WB on Mouse tumor at: 1:1000 (Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate).| Product Name | GATA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GATA4, ASD2, VSD1, TACHD |
| Product description | GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. GATA4is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects.1) Anttonen, M., et al., (2005) J. Clin. Endocrinol. Metab. 90 (12), 6529-6535. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GATA4 antibody can be used for detection of GATA4 by ELISA at 1:1562500. GATA4 antibody can be used for detection of GATA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GATA4 |
|---|---|
| Alternative Names | GATA4 ASD2 VSD1 TACHD |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in WB on Mouse tumor at: 1:1000 (Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | GATA4 antibody can be used for detection of GATA4 by ELISA at 1:1562500. GATA4 antibody can be used for detection of GATA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GATA4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
