Application
Confocal immunofluorescent analysis of GBE1 Antibody with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of GBE1 Antibody in HL-60 cell line lysates (35ug/lane).Application
Western blot analysis of GBE1 Antibody in mouse liver tissue lysates (35ug/lane)| Product Name | GBE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GBE1 is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease).1) Konstantinidou, A.E., et al. Placenta 29(4):378-381(2008) |
| Immunogen | This GBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-556 amino acids from the Central region of human GBE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GBE1 |
|---|---|
| Alternative Names | 4-alpha-glucan-branching enzyme Brancher enzyme Glycogen-branching enzyme GBE1 |
| Molecular Weight(MW) | 80 kDa |
Application
Confocal immunofluorescent analysis of GBE1 Antibody with HepG2 cell followed by Alexa Fluor? 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of GBE1 Antibody in HL-60 cell line lysates (35ug/lane).
Application
Western blot analysis of GBE1 Antibody in mouse liver tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
