Application
Immunohistochemistry staining of GBL in skin tissue using GBL Antibody.| Product Name | GBL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLST8, G protein beta subunit-like, GbetaL, Gable, LST8, POP3, TORC subunit LST8, GBL, Protein GbetaL, WAT1 |
| Product description | LST8, a 36-40 kDa intracellular protein, is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis. Part of the mammalian target of rapamycin complex 1 (mTORC1), which is composed of mTOR, regulatory associated protein of mTOR (Raptor), mammalian LST8/G-protein β-subunit like protein (mLST8/GβL) and the recently identified partner PRAS40. This complex is characterized by the classic features of mTOR by functioning as a nutrient/energy/redox sensor and controlling protein synthesis. The activity of this complex is stimulated by insulin, growth factors, serum, phosphatidic acid, amino acids (particularly leucine), and oxidative stress. |
| Immunogen | A portion of amino acids 150-200 of human LST8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| GBL antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
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| Species Reactivity | BovineChickenChimpanzeeDogHumanMacaque MonkeyMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | MLST8 |
|---|---|
| Alternative Names | MLST8 G protein beta subunit-like GbetaL Gable LST8 POP3 TORC subunit LST8 GBL Protein GbetaL WAT1 |
Application
Immunohistochemistry staining of GBL in skin tissue using GBL Antibody.| Application Notes | GBL antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | GBL antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.2% gelatin, 0.05% sodium azide. |
