Application
Immunocytochemistry of GBP6 in HeLa cells with GBP6 antibody at 2.5 μg/mL.Application
Immunofluorescence of GBP6 in HeLa cells with GBP6 antibody at 5 μg/mL.Application
Western blot analysis of GBP6 in Hela cell lysate with GBP6 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | GBP6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GBP6 Antibody: Guanylate-binding protein 6, GTP-binding protein 6, GBP-6 |
| Product description | GBP6 Antibody: The guanylate-binding proteins (GBPs), a family of interferon-induced GTP-binding proteins function in innate immunity against microbial and viral pathogens. In humans, there are seven GBPs from hGBP1 to hGBP7. Human GBP1 and GBP2 have been shown to exhibit antiviral activity as well as being able to regulate the inhibition of proliferation and invasion of endothelial cells in response to interferon (IFN) (1,3). GBP6 is induced by IFN and hydrolyzes GTP to both GDP and GMP. The precise function of GBP6 is still under investigation.1) Vestal DJ. The guanylate-binding proteins (GBPs): proinflammatory cytokine-induced members of the dynamin superfamily with unique GTPase activity. J. Interferon Cytokine Res. 2005; 25:435-43. |
| Immunogen | GBP6 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human GBP6. The immunogen is located within the last 50 amino acids of GBP6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| GBP6 antibody can be used for detection of GBP6 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GBP6 |
|---|---|
| Alternative Names | GBP6 Antibody: Guanylate-binding protein 6 GTP-binding protein 6 GBP-6 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | GBP6 antibody is predicted to not cross-react with other GBP family members. |
Application
Immunocytochemistry of GBP6 in HeLa cells with GBP6 antibody at 2.5 μg/mL.
Application
Immunofluorescence of GBP6 in HeLa cells with GBP6 antibody at 5 μg/mL.
Application
Western blot analysis of GBP6 in Hela cell lysate with GBP6 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | GBP6 antibody can be used for detection of GBP6 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GBP6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GBP6 Antibody is supplied in PBS containing 0.02% sodium azide. |
