Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GDF5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect GDF5 in A549 cell lysate.| Product Name | GDF5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GDF5 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development.1) Kusafuka, K., et al., Virchows Arch. 442(5):482-490 (2003). |
| Immunogen | This GDF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-41 amino acids from the N-terminal region of human GDF5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | GDF5 |
|---|---|
| Alternative Names | Growth/differentiation factor 5 GDF-5 Bone morphogenetic protein 14 BMP-14 Cartilage-derived morphogenetic protein 1 CDMP-1 Lipopolysaccharide-associated protein 4 LAP-4 LPS-associated protein 4 Radotermin GDF5 BMP14 CDMP1 |
| Molecular Weight(MW) | 55 kDa |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GDF5 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect GDF5 in A549 cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
