Application
Western blot analysis of GEMC1 in mouse heart tissue lysate with GEMC1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | GEMC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GEMC1 Antibody: GEMC1, GEMC1, Geminin coiled-coil domain-containing protein 1 |
| Product description | GEMC1 Antibody: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.1) Piergiovanni G, Costanzo V. GEMC1 is a novel TopBP1-interacting protein involved in chromosomal DNA replication. Cell Cycle 2010; 9:3662-6. |
| Immunogen | GEMC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human GEMC1. The immunogen is located within amino acids 130 - 180 of GEMC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GEMC1 |
|---|---|
| Alternative Names | GEMC1 Antibody: GEMC1 GEMC1 Geminin coiled-coil domain-containing protein 1 |
| Entrez Gene | 647309 |
|---|---|
| Protein Accession | NP_001140158 |
Application
Western blot analysis of GEMC1 in mouse heart tissue lysate with GEMC1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | GEMC1 antibody can be used for detection of GEMC1 by Western blot at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GEMC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GEMC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
