Application
Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.Application
Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.| Product Name | GFR alpha 2 Antibody |
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| Antibody Type | Primary Antibodies |
| Product description | GFR alpha 2 Antibody: Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFRβ, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFRα-2 recently. GFRα-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFRα-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.1) Kotzbauer PT, Lampe PA, Heuckeroth RO, et al. Neurturin, a relative glial-cell-line-derived neurotrophic factor. Nature 1996; 384:467-70. |
| Immunogen | GFR alpha 2 antibody was raised against a 15 amino acid peptide near the center of human GFR alpha 2. The immunogen is located within amino acids 360 - 410 of GFR alpha 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GFRA2 |
|---|---|
| Alternative Names | GFR alpha 2 Antibody: NTNRA RETL2 TRNR2 GDNFRB NRTNR-ALPHA GDNF family receptor alpha-2 GDNF receptor beta GDNF receptor alpha-2 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of GFR alpha 2 in HeLa cells with GFR alpha 2 antibody at 5 μg/mL.
Application
Western blot analysis of GFR alpha 2 in HeLa total cell lysate with GFR alpha 2 antibody at 1 μg/mL.| Application Notes | GFR alpha 2 antibody can be used for detection of GFR alpha 2 by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | GFR alpha 2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GFR alpha 2 Antibody is supplied in PBS containing 0.02% sodium azide. |
