| Product Name | GITRL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glucocorticoid-induced TNF Receptor Ligand; AITRL; Activation-inducible TNF-related Ligand; TNFSF18 |
| Product description | GITRL is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e. g. testis, . ). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T-cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors. |
| Immunogen | Recombinant human GITRL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: (direct and indirect: 1:5,000-1:10,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TNFSF18 |
|---|---|
| Alternative Names | Glucocorticoid-induced TNF Receptor Ligand AITRL Activation-inducible TNF-related Ligand TNFSF18 |
| Tissue Specificity | Recognizes human GITRL. Detects a band of ~15-17kDa by Western blot. Does not cross-react with mouse GITRL. |
| Application Notes | ELISA: (direct and indirect: 1:5,000-1:10,000). Western Blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for at least 6 months after receipt when stored at -20˚C. |
| Storage Buffer | Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives. |
