Application
Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 μg/mL.Application
Western blot analysis of Gle1 in 293 cell lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 20 μg/mL.| Product Name | Gle1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gle1 Antibody: LCCS, GLE1L, LCCS1, hGLE1, Nucleoporin GLE1, GLE1-like protein |
| Product description | Gle1 Antibody: The proper expression of gene products in eukaryotic cells relies on efficient transport of mRNA molecules out of the nucleus. Gle1 is an essential mRNA export factor in both human and yeast cells. It associates with the nuclear pore complex (NPC) through hCG1 and NUP155 in mammalian cells and in conjunction with inositol hexakisphosphate (IP6), stimulates Dbp5, a member of the DEAD-box helicase family, triggering mRNP remodeling and facilitating RNA export from the nucleus. Recent evidence suggests that mutations in Gle1 causing defects in mRNA export can result in human disease. At least three isoforms of Gle1 are known to exist.1) Murphy R and Wente SR. An RNA-export mediator with an essential nuclear export signal. Nature1996; 383:357-60. |
| Immunogen | Gle1 antibody was raised against a 14 amino acid synthetic peptide near the center of human Gle1. The immunogen is located within amino acids 390 - 440 of Gle1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLE1 |
|---|---|
| Alternative Names | Gle1 Antibody: LCCS GLE1L LCCS1 hGLE1 Nucleoporin GLE1 GLE1-like protein |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Entrez Gene | 2733 |
|---|---|
| Protein Accession | NP_001003722 |
Application
Immunohistochemistry of Gle1 in mouse brain tissue with Gle1 antibody at 2.5 μg/mL.
Application
Western blot analysis of Gle1 in 293 cell lysate with Gle1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of gle1 in mouse brain tissue with gle1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | Gle1 antibody can be used for detection of Gle1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Gle1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Gle1 Antibody is supplied in PBS containing 0.02% sodium azide. |
