Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Product Name | GLE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLE1, GLE1L, hGLE1, LCCS, LCCS1 |
| Product description | GLE1 is a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly (A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly (A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly (A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly (A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.1) Nousiainen, H.O., (2008) Nat. Genet. 40 (2), 155-157. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GLE1 antibody can be used for detection of GLE1 by ELISA at 1:1562500. GLE1 antibody can be used for detection of GLE1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLE1 |
|---|---|
| Alternative Names | GLE1 GLE1L hGLE1 LCCS LCCS1 |
| Molecular Weight(MW) | 75 kDa |
Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | GLE1 antibody can be used for detection of GLE1 by ELISA at 1:1562500. GLE1 antibody can be used for detection of GLE1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GLE1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
