The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).1) Grenningloh, G., et al., (1990) EMBO J. 9:771-776..
Immunogen
Antibody produced in rabbits immunized with a synthetic phosphopeptide corresponding a region of human GLRA1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
GLRA1 antibody can be used for detection of GLRA1 by ELISA at 1:62500. GLRA1 antibody can be used for detection of GLRA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Western blot analysis of GLRA1 in Raji Cell Lysate using GLRA1 antibody at 1 μg/mL (12% gel).
Positive Control
1) Cat. No. 1207 - Raji Cell Lysate
Application Notes
GLRA1 antibody can be used for detection of GLRA1 by ELISA at 1:62500. GLRA1 antibody can be used for detection of GLRA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GLRA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt