Application
Glycine Receptor Antibody| Product Name | Glycine Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GLYRA1, Glyr, Glycine receptor 48 kDa subunit, |
| Product description | Gamma-amGlycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission. The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin. Gene targeting in mice showed that gephyrin is required for synaptic clustering of glycine receptors in spinal cord. The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins. These receptors have also been extremely useful in studies of synaptic clustering of receptors. During postnatal motoneuron development, the glycine receptor alpha subunit changes from alpha2 (fetal) to alpha1 (adult).1) Schofield, C.M., Jenkins, A. and Harrison, N.L., "A highly conserved aspartic acid residue in the signature disulfide loop of the a1 subunit is a determinant of gating in the glycine receptor," J. Biol. Chem. 278 (2003) 34,079-34,083. |
| Immunogen | Glycine Receptor polyclonal antibody was raised against a peptide derived from the amino terminus of the *1 subunit of Glycine Receptor. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB). Rabbit anti-Glycine Receptor recognizes glycine receptor α1 and α2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
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| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Glra1 |
|---|---|
| Alternative Names | GLYRA1 Glyr Glycine receptor 48 kDa subunit |
| Molecular Weight(MW) | 48 |
Application
Glycine Receptor Antibody| Application Notes | Glycine Receptor antibody is specific immunolabeling of the ~48k *1 and *2 subunits of the receptor in Western blots of rat spinal cord and in cell extracts containing the recombinant human glycine receptors. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen derived from the amino terminus of the *1 subunit of the receptor. Applications include Dot Blots (DB) and Western Blots (WB). Rabbit anti-Glycine Receptor recognizes glycine receptor α1 and α2 in rat spinal cord and brain stem. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Glycine Receptor antibody can be stored at -20˚C. After reconstitution in 50 μl PBS, the antibody should be aliquot and stored at -20˚C. This product is stable at -20˚C for at least 1 year. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
