Application
Immunofluorescence of GNPDA2 in mouse kidney tissue with GNPDA2 antibody at 20 μg/mL.| Product Name | GNPDA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GNPDA2 Antibody: GNP2, SB52, GNP2, Glucosamine-6-phosphate isomerase 2, Glucosamine-6-phosphate deaminase 2, GNPDA 2 |
| Product description | GNPDA2 Antibody: Glucosamine-6-phosphate deaminase (GNPDA) is an allosteric enzyme that catalyzes the reversible conversion of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium. GNPDA2 is the second mammalian glucosamine-6-phosphate deaminase discovered, and is closer in structure and activity to the E. coli enzyme than GNPDA1. GNPDA1 possesses greater affinity for ammonium than either GNPDA2 or the E. coli enzyme suggesting that the forward reaction of D-glucosamine-6-phosphate into D-fructose-6-phosphate and ammonium is catalyzed at a slower rate than GNPDA2.1) Wolosker H, Kline D, Bian Y, et al. Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity. FASEB J.1998; 12:91-9. |
| Immunogen | GNPDA2 antibody was raised against an 8 amino acid synthetic peptide near the carboxy terminus of human GNPDA2. The immunogen is located within the last 50 amino acids of GNPDA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GNPDA2 |
|---|---|
| Alternative Names | GNPDA2 Antibody: GNP2 SB52 GNP2 Glucosamine-6-phosphate isomerase 2 Glucosamine-6-phosphate deaminase 2 GNPDA 2 |
| Sequence Similarities | 2) Cat. No. 17-010 - 293 Cell Slide |
| Tissue Specificity | GNPDA2 antibody is predicted to not cross-react with GNPDA1. |
Application
Immunofluorescence of GNPDA2 in mouse kidney tissue with GNPDA2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
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| Application Notes | GNPDA2 antibody can be used for detection of GNPDA2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GNPDA2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GNPDA2 Antibody is supplied in PBS containing 0.02% sodium azide. |
