Application
Immunohistochemistry of GOLGA5 in human testis tissue with GOLGA5 antibody at 5 μg/mL.Application
Immunofluorescence of GOLGA5 in human testis tissue with GOLGA5 antibody at 20 μg/mL.Application
Western blot analysis of GOLGA5 in human testis tissue lysate with GOLGA5 antibody at 1 μg/mL.| Product Name | GOLGA5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein |
| Product description | GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).1) Bascom RA, Srinivasan S, Nussbaum RL. Identification and characterization of golgin 84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. J. Biol. Chem. 1999; 274:2953-62. |
| Immunogen | GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5. The immunogen is located within amino acids 540 - 590 of GOLGA5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOLGA5 |
|---|---|
| Alternative Names | GOLGA5 Antibody: RFG5 GOLIM5 ret-II RETII RFG5 PIG31 Golgin subfamily A member 5 Cell proliferation-inducing gene 31 protein |
| Molecular Weight(MW) | Predicted: 64, 80 kDa Observed: 65, 78 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events. |
Application
Immunohistochemistry of GOLGA5 in human testis tissue with GOLGA5 antibody at 5 μg/mL.
Application
Immunofluorescence of GOLGA5 in human testis tissue with GOLGA5 antibody at 20 μg/mL.
Application
Western blot analysis of GOLGA5 in human testis tissue lysate with GOLGA5 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GOLGA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide. |
