Application
Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | GOLPH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GOLPH1 Antibody: PAP7, GCP60, GOCAP1, GOLPH1, Golgi resident protein GCP60, Acyl-CoA-binding domain-containing protein 3 |
| Product description | GOLPH1 Antibody: GOLPH1, also known as GCP60, was initially identified as a Golgi protein that can interact with the integral membrane protein giantin and is thought to be involved in the maintenance of the Golgi structure. GOLPH1 has also been shown to interact with other Golgi proteins such as Golgin-160, a Golgi protein that can be cleaved by caspases-2, -3, and -7, leading to the nuclear localization of Golgin-160. GOLPH1 interaction with the Golgin-160 fragments is stronger than that with the intact Golgin-160, with its interaction regulated by the oxidation state of Cys-463 within GOLPH1, suggesting that the nuclear localization of the caspase-cleaved Golgin-160 fragments is a highly coordinated event. GOLPH1 has also been found to interact with Numb, a cytosolic signaling protein that mediates asymetric cell division of neural progenitor cells to a daughter progenitor cell and a neuron, suggesting that Golgi fragmentation and reconstitution during the cell cycle differentially regulate Numb signaling through changes in GOLPH1 subcellular distribution and may couple cell fate with cell cycle progression.1) Sohda M, Misumi Y, Yamamoto A, et al. Identification and characterization of a novel Golgi protein, GCP60, that interacts with the integral membrane protein giantin. J. Biol. Chem.2001; 276:45298-306. |
| Immunogen | GOLPH1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human GOLPH1. The immunogen is located within amino acids 50 - 100 of GOLPH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GOLPH1 antibody can be used for detection of GOLPH1 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACBD3 |
|---|---|
| Alternative Names | GOLPH1 Antibody: PAP7 GCP60 GOCAP1 GOLPH1 Golgi resident protein GCP60 Acyl-CoA-binding domain-containing protein 3 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (83%) |
Application
Western blot analysis of GOLPH1 in K562 cell lysate with GOLPH1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | GOLPH1 antibody can be used for detection of GOLPH1 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GOLPH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GOLPH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
