Application
Western blot analysis of 5 ng of gp120 with gp120 antibody at 1 μg/mL.| Product Name | gp120 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | gp120 Antibody: Envelope glycoprotein gp160 |
| Product description | gp120 Antibody: Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.1) Pinter A. Roles of HIV-1 Env variable regions in viral neutralization and vaccine development. Curr. HIV Res.2007; 5:542-53. |
| Immunogen | gp120 antibody was raised against 16 amino acid synthetic peptide representing the major HIV-1 consensus sequence near the N-terminal region of V1/V2 loop. The immunogen is located within amino acids 80 - 130 of gp120. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Gp120 antibody can be used for detection of gp120 by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | env |
|---|---|
| Alternative Names | gp120 Antibody: Envelope glycoprotein gp160 |
| Molecular Weight(MW) | Predicted: 120 kDa Observed: 120 kDa |
Application
Western blot analysis of 5 ng of gp120 with gp120 antibody at 1 μg/mL.| Application Notes | Gp120 antibody can be used for detection of gp120 by Western blot at 1 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | gp120 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | gp120 Antibody is supplied in PBS containing 0.02% sodium azide. |
