Application
Antibody used in IHC on Human kidney.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | GPAA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPAA1, GAA1, hGAA1 |
| Product description | Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. GPAA1 presumably functions in GPI anchoring at the GPI transfer step. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.1) Vainauskas, S. (2005) J. Biol. Chem. 280 (16), 16402-16409. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPAA1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| GPAA1 antibody can be used for detection of GPAA1 by ELISA at 1:62500. GPAA1 antibody can be used for detection of GPAA1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPAA1 |
|---|---|
| Alternative Names | GPAA1 GAA1 hGAA1 |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in IHC on Human kidney.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GPAA1 antibody can be used for detection of GPAA1 by ELISA at 1:62500. GPAA1 antibody can be used for detection of GPAA1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GPAA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
