Application
Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.Application
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.Application
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.| Product Name | GPAT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPAT1 Antibody: GPAT, GPAT1, KIAA1560, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT-1 |
| Product description | GPAT1 Antibody: Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets.1) Coleman RA and Lee DP. Enzymes of triacylglycerol synthesis and their regulation. Prog. Lipid Res. 2004; 43:134-76. |
| Immunogen | GPAT1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAT1. The immunogen is located within amino acids 730 - 780 of GPAT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPAM |
|---|---|
| Alternative Names | GPAT1 Antibody: GPAT GPAT1 KIAA1560 Glycerol-3-phosphate acyltransferase 1 mitochondrial GPAT-1 |
| Molecular Weight(MW) | Predicted: 87, 91 kDa Observed: 92 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Pig: (100%) |
| Tissue Specificity | At least four isoforms of GPAT1 are known to exist; this antibody will detect only the two largest isoforms. |
Application
Immunohistochemistry of GPAT1 in mouse brain tissue with GPAT1 antibody at 5 μg/ml.
Application
Immunohistochemistry of GPAT1 in rat brain with GPAT1 antibody at 2.5 μg/mL.
Application
Western blot analysis of GPAT1 in rat brain tissue lysate with GPAT1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of GPAT1 in Rat Brain tissue with GPAT1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | GPAT1 antibody can be used for detection of GPAT1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GPAT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GPAT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
