Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome.1) Boily, G., et al., (2004) Br. J. Cancer 90 (8), 1606-1611.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPC3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GPC3 antibody can be used for detection of GPC3 by ELISA at 1:1562500. GPC3 antibody can be used for detection of GPC3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Placenta at 2.5 ug/ml.
Positive Control
1) Cat. No. 1309 - Human Placenta Lysate
Application Notes
GPC3 antibody can be used for detection of GPC3 by ELISA at 1:1562500. GPC3 antibody can be used for detection of GPC3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GPC3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt