Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).Application
GPLD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | GPLD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with polipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPLD1, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.1) Tsang, T.C., et al., FASEB J. 6, A1922-A1922 (1992). |
| Immunogen | This GPLD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 679-709 amino acids from the C-terminal region of human GPLD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GPLD1 |
|---|---|
| Alternative Names | Phosphatidylinositol-glycan-specific phospholipase D PI-G PLD Glycoprotein phospholipase D Glycosyl-phosphatidylinositol-specific phospholipase D GPI-PLD GPI-specific phospholipase D GPLD1 PIGPLD1 |
| Molecular Weight(MW) | 92 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPLD2 antibody detected the GPLD2 protein (arrow).
Application
GPLD1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
