GPR15 Antibody: Human immunodeficiency virus (HIV) and related viruses require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four other molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. GPR15, also known as BOB, is a G protein-coupled receptor that serves as coreceptor for the simian immunodeficiency virus (SIV), and for strains of HIV-2 and M-tropic HIV-1.1) Heiber M, Marchese A, Nguyen T, et al. A novel human gene encoding a G-protein-coupled receptor (GPR15) is located on chromosome 3. Genomics 1996; 32:462-5
Immunogen
GPR15 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human GPR15. The immunogen is located within the first 50 amino acids of GPR15.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ELISAICCWB
GPR15 antibody can be used for detection of GPR15 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.:
Western blot analysis of GPR15 in human spleen lysate with GPR15 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunocytochemistry of GPR15 in A431 cells with GPR15 antibody at 5 μg/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
GPR15 antibody can be used for detection of GPR15 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.:
Additional Information
Form
Liquid
Storage Instructions
GPR15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
GPR15 Antibody is supplied in PBS containing 0.02% sodium azide.