Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
GPR17 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of GPR17 Antibody in MDA-MB435 cell line lysates (35ug/lane)| Product Name | GPR17 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Uracil nucleotide/cysteinyl leukotriene receptor, UDP/CysLT receptor, G-protein coupled receptor 17, P2Y-like receptor, R12, GPR17 |
| Product description | Members of the G protein coupled receptor (GPCR) superfamily contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. The organization of the GPR17 gene differs from that of many other GPCRs in that the open reading frame is distributed on 2 exons; an additional exon contains the 5 prime untranslated region. Human GPR17 is expressed as 2.3 and 6.3 kb mRNAs exclusively in brain. The 2 transcripts appear to represent alternatively polyadenylated variants. Based on protein sequence homology and the conservation of certain key residues, GPR17 appears to be closely related to the P2Y family of GPCRs. There are two nemed isoforms.1) Pugliese, A.M., et al. Am. J. Physiol., Cell Physiol. 297 (4), C1028-C1040 (2009) |
| Immunogen | This GPR17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the Central region of human GPR17. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR17 |
|---|---|
| Alternative Names | Uracil nucleotide/cysteinyl leukotriene receptor UDP/CysLT receptor G-protein coupled receptor 17 P2Y-like receptor R12 GPR17 |
| Molecular Weight(MW) | 41 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
GPR17 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of GPR17 Antibody in MDA-MB435 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
