Application
Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
GPR45 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy for immunohistochemistry.| Product Name | GPR45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable G-protein coupled receptor 45, PSP24-1, PSP24-alpha, GPR45 |
| Product description | This intronless gene encodes a member of the G protein-coupled receptor (GPCR) family. Members of this protein family contain seven putative transmembrane domains and may mediate signaling processes to the interior of the cell via activation of heterotrimeric G proteins. This protein may function in the central nervous system.1) Salonen, J.T., et al. Am. J. Hum. Genet. 81(2):338-345(2007) Wu, C., et al. Proteomics 7(11):1775-1785(2007) Hillier, L.W., et al. Nature 434(7034):724-731(2005) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):957-964(2000) Kawasawa, Y., et al. Biochem. Biophys. Res. Commun. 276(3):952-956(2000) |
| Immunogen | This GPR45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-149 amino acids from the Central region of human GPR45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPR45 |
|---|---|
| Alternative Names | Probable G-protein coupled receptor 45 PSP24-1 PSP24-alpha GPR45 |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:2000 dilution + human cerebellum lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
GPR45 Antibdy immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPR45 Antibdy for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
