Application
Immunofluorescence of GPRASP1 in human breast carcinoma tissue with GPRASP1 antibody at 20 μg/ml.Application
Immunohistochemistry of GPRASP1 in human breast carcinoma tissue with GPRASP12 antibody at 5 μg/ml.Application
Western blot analysis of GPRASP1 in A-20 cell lysate with GPRASP1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | GPRASP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G-protein coupled receptor-associated sorting protein 1, GASP, GASP-1, GASP1 |
| Product description | The G protein-coupled receptor associated sorting protein 1 (GPRASP1) is a member of the GPRASP family and is thought to modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors (1,2). GPRASP1 interacts with receptors such as the D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CNR1) and targets these proteins for degradation in lysosomes (2). Recently, it has been shown that increased GPRASP1 levels may act as a potential biomarker for breast cancer (3).1) Simonin F, Karcher P, Boeuf JJ, et al. Identification of a novel family of G protein-coupled receptor associated proteins. J. Neurochem. 2004; 89:766-75. |
| Immunogen | GPRASP1 antibody was raised against a 15 amino acid peptide near the amino terminus of human GPRASP1. The immunogen is located within amino acids 260 - 310 of GPRASP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPRASP1 |
|---|---|
| Alternative Names | G-protein coupled receptor-associated sorting protein 1 GASP GASP-1 GASP1 |
| Molecular Weight(MW) | Predicted: 153 kDa Observed: 150 kDa |
| Tissue Specificity | GPRASP1 antibody is human and mouse reactive. This antibody is predicted to not cross-react with GPRASP2. |
| Entrez Gene | 9737 |
|---|---|
| Protein Accession | NP_001171656 |
Application
Immunofluorescence of GPRASP1 in human breast carcinoma tissue with GPRASP1 antibody at 20 μg/ml.
Application
Immunohistochemistry of GPRASP1 in human breast carcinoma tissue with GPRASP12 antibody at 5 μg/ml.
Application
Western blot analysis of GPRASP1 in A-20 cell lysate with GPRASP1 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | GPRASP1 antibody can be used for detection of GPRASP1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GPRASP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GPRASP1 antibody is supplied in PBS containing 0.02% sodium azide. |
