GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as
alanine transaminases, are pyridoxal enzymes that catalyze the
reversible transamination between alanine and 2-oxoglutarate to
form pyruvate and glutamate. By mediating the conversion of these 4
major intermediate metabolites, these transaminases have roles in
gluconeogenesis and in amino acid metabolism.1) Glinghammar, B., et al. Int. J. Mol. Med. 23(5):621-631(2009)
Immunogen
This GPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-44 amino acids from the N-terminal region of human GPT2.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.