Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Product Name | GPX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GPX3, GPx-P, GSHPx-3, GSHPx-P |
| Product description | GPX3 belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination.Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme. GPX3 expression appears to be tissue-specific.1) Lee, O.J., (2005) Neoplasia 7 (9), 854-861. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GPX3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| GPX3 antibody can be used for detection of GPX3 by ELISA at 1:312500. GPX3 antibody can be used for detection of GPX3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GPX3 |
|---|---|
| Alternative Names | GPX3 GPx-P GSHPx-3 GSHPx-P |
| Molecular Weight(MW) | 25 kDa |
Application
Antibody used in WB on Human HepG2 cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | GPX3 antibody can be used for detection of GPX3 by ELISA at 1:312500. GPX3 antibody can be used for detection of GPX3 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store GPX3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
