Application
Immunohistochemical staining of human brain tissue using Grik1 antibody at 2.5 μg/mL.Application
Immunofluorescence of Grik1 in mouse brain tissue with Grik1 Antibodyat 20 μg/mL.Application
Immunohistochemistry of Grik1 in mouse brain tissue with Grik1 Antibodyat 5 μg/mL.Application
Immunofluorescence of Grik1 in Human Brain cells with Grik1 antibody at 20 μg/mL.Application
Western blot analysis of Grik1 in P815 cell lysate with Grik1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | Grik1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Grik1 Antibody: EAA3, EEA3, GLR5, GLUR5, GluK1, Glutamate receptor ionotropic, kainate 1, Excitatory amino acid receptor 3 |
| Product description | Grik1 Antibody: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. Grik1, also known as glutamate receptor 5, belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. Grik1 is expressed in GABAergic interneurons of the hippocampus and are thought to participate in the formation of various subtypes of kainate receptors with Grik2 and KA2. Stimulation of Grik1 leads to intracellular calcium release and activation of protein kinase C. Excessive activation has been associated with psychiatric, neurological and neurodegenerative diseases. Numerous isoforms of Grik1 are known to exist and may be subject to RNA editing within the second transmembrane domain, which is thought to alter the properties of ion flow.1) Tanaka K. Functions of glutamate transports in the brain. Neurosci. Res.2000; 37:15-9. |
| Immunogen | Grik1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human Grik1. The immunogen is located within the last 50 amino acids of Grik1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Grik1 antibody can be used for detection of Grik1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRIK1 |
|---|---|
| Alternative Names | Grik1 Antibody: EAA3 EEA3 GLR5 GLUR5 GluK1 Glutamate receptor ionotropic kainate 1 Excitatory amino acid receptor 3 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemical staining of human brain tissue using Grik1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Grik1 in mouse brain tissue with Grik1 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of Grik1 in mouse brain tissue with Grik1 Antibodyat 5 μg/mL.
Application
Immunofluorescence of Grik1 in Human Brain cells with Grik1 antibody at 20 μg/mL.
Application
Western blot analysis of Grik1 in P815 cell lysate with Grik1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1286 - P815 Cell Lysate |
|---|---|
| Application Notes | Grik1 antibody can be used for detection of Grik1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Grik1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Grik1 Antibody is supplied in PBS containing 0.02% sodium azide. |
