Application
Western blot analysis of GRK6 in rat small intestine tissue lysate with GRK6 antibody at 1 μg/ml.Application
Immunofluorescence of GRK6 in human small intestine tissue with GRK6 antibody at 20 μg/mL.Application
Immunohistochemistry of GRK6 in human small intestine tissue with GRK6 antibody at 5 μg/mL.| Product Name | GRK6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRK6 Antibody: GPRK6, GPRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6 |
| Product description | The G protein–coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).1) Inglese J, Freedman NJ, Koch WJ, et al. Structure and mechanism of the G protein-coupled receptor kinases. J. Biol. Chem. 1993; 268:23735-8. |
| Immunogen | GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6. The immunogen is located within the last 50 amino acids of GRK6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRK6 |
|---|---|
| Alternative Names | GRK6 Antibody: GPRK6 GPRK6 G protein-coupled receptor kinase 6 G protein-coupled receptor kinase GRK6 |
| Molecular Weight(MW) | Predicted: 63 kDa Observed: 63 kDa |
| Sequence Similarities | 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide |
| Tissue Specificity | GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family. |
| Entrez Gene | 2870 |
|---|---|
| Protein Accession | NP_001004106 |
Application
Western blot analysis of GRK6 in rat small intestine tissue lysate with GRK6 antibody at 1 μg/ml.
Application
Immunofluorescence of GRK6 in human small intestine tissue with GRK6 antibody at 20 μg/mL.
Application
Immunohistochemistry of GRK6 in human small intestine tissue with GRK6 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1468 - Rat Small Intestine Tissue Lysate |
|---|---|
| Application Notes | GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GRK6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | GRK6 antibody is supplied in PBS containing 0.02% sodium azide. |
