Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-GRK7 Antibody in HepG2 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | GRK7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G protein-coupled receptor kinase 7, G protein-coupled receptor kinase GRK7, GRK7, GPRK7 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).1) Weiss, E.R., et al., J. Neurosci. 21(23):9175-9184 (2001). |
| Immunogen | This GRK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-538 amino acids from the C-terminal region of human GRK7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRK7 |
|---|---|
| Alternative Names | G protein-coupled receptor kinase 7 G protein-coupled receptor kinase GRK7 GRK7 GPRK7 |
| Molecular Weight(MW) | 62 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-GRK7 Antibody in HepG2 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
