Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.Application
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human GRO-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human GRO-gamma (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-gamma.| Product Name | Gro-gamma Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GRO3, GROg, MIP2B, SCYB3, MIP-2b, CINC-2b, GRO3, GROG, C-X-C motif chemokine 3, Growth-regulated protein gamma, GRO-gamma |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGRO-gamma (human GRO-gamma). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0 - 2.0 μg/mL of this antibody is required. ELISA: To detect hGRO-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO-gamma. Sandwich: To detect hGRO-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-γ (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-γ. Western Blot: To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCL3 |
|---|---|
| Alternative Names | GRO3 GROg MIP2B SCYB3 MIP-2b CINC-2b GRO3 GROG C-X-C motif chemokine 3 Growth-regulated protein gamma GRO-gamma |
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/ml-0.5mg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Application
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human GRO-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human GRO-gamma (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human GRO-gamma.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hGRO-gamma (100 ng/mL), a concentration of 1.0 - 2.0 μg/mL of this antibody is required. ELISA: To detect hGRO-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hGRO-gamma. Sandwich: To detect hGRO-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human GRO-γ (XP-5150Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGRO-γ. Western Blot: To detect hGRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGRO-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Gro-gamma antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
