Application
Immunocytochemistry of GRTP1 in K562 cells with GRTP1 antibody at 2.5 μg/mL.Application
Western blot analysis of GRTP1 in K562 cell lysate with GRTP1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of GRTP1 in K562 cells with GRTP1 antibody at 20 μg/mL.| Product Name | GRTP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | GRTP1 Antibody: Growth hormone regulated TBC protein 1 (GRTP1) was identified through microarray analysis as a gene whose expression is regulated by growth hormone (GH). It contains the TBC signature motif of GTPase activator proteins of Rab-like small GTPases. GRTP1 mRNA is expressed at highest levels in testes, and is also present in kidney, lung, liver, and intestine. Administration of GH to mice resulted in an increase in GRTP1 mRNA in testes, but a decrease of GRTP1 mRNA in kidney and liver. GRTP1 was identified as an HIV dependency factor (HDF), suggesting that GRTP1 may be an important drug target in HIV treatment. At least three isoforms of GRTP1 are known to exist; this antibody should recognize all isoforms.1) Lu C, Kasik J, Stephan DA, et al. Grtp1, a novel gene regulated by growth hormone. Endocriniology2001; 142:4568-71. |
| Immunogen | GRTP1 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human GRTP1. The immunogen is located within the first 50 amino acids of GRTP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| GRTP1 antibody can be used for detection of GRTP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GRTP1 |
|---|---|
| Alternative Names | GRTP1 Antibody: TBC1D6 TBC1D6 Growth hormone-regulated TBC protein 1 TBC1 domain family member 6 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Immunocytochemistry of GRTP1 in K562 cells with GRTP1 antibody at 2.5 μg/mL.
Application
Western blot analysis of GRTP1 in K562 cell lysate with GRTP1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of GRTP1 in K562 cells with GRTP1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | GRTP1 antibody can be used for detection of GRTP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | GRTP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | GRTP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
