Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Daudi whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | GSG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase, GSG2 |
| Product description | Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Mitotic phosphorylation of H3 Thr 3 occurs in prophase and dephosphorylation during anaphase. Haspin, a dual serine/threonine kinase, plays an important role in regulation of chromosome and spindle function during mitosis and meiosis via its function in phosphorylation of the threonine residue in the third position of histone 3 (Thr3).1) Dai, Jun et al. Genes Dev 19:472-88 (2005). |
| Immunogen | This Haspin GSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323~352 amino acids from the central region of human haspin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GSG2 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase haspin Germ cell-specific gene 2 protein H-haspin Haploid germ cell-specific nuclear protein kinase GSG2 |
| Molecular Weight(MW) | 88 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Daudi whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
