Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.Application
Immunohistochemical analysis of paraffin - embedded human lung carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.Application
Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.| Product Name | GSK3alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSK-3 alpha, |
| Product description | GSK3 alpha (Glycogen synthase kinase 3 alpha), with 483-amino acid protein (about 53 kDa), belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily,GSK3 subsubfamily, which also includes GSK3 beta.GSK3 is a multifunctional serine/threonine kinase that is usually inactivated by serine phosphorylation in response to extracellular cues. GSK3 is a key regulator of numerous signalling pathways, including cellular responses to Wnt, receptor tyrosine kinases and G-protein-coupled receptors and is involved in a wide range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation.GSK3 alpha is implicated in the control of several regulatory proteins including glycogen synthase and transcription factors.1) Bradley W. Doble and James R. Woodgett. J. Cell Sci., Apr 2003; 116: 1175. |
| Immunogen | Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GSK3A |
|---|---|
| Alternative Names | GSK-3 alpha |
Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.
Application
Immunohistochemical analysis of paraffin - embedded human lung carcinoma showing cytoplasmic localization using GSK3 alpha antibody with DAB staining.
Application
Western blot analysis using anti - human GSK3 alpha nonoclonal antibody against truncated GSK3 alpha recombinant protein and A549 lysate.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GSK3 alpha monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
