Application
Immunofluorescense analysis hof Hela cells using GSK3a antibody showing cytoplasmic localizaiton.Application
Western blot analysis using GSK3 monoclonal antibody against truncated GSK3alpha recombinant protein| Product Name | GSK3alpha Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GSK-3 alpha, |
| Product description | Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.1) Mendez P, Garcia-Segura LM. Endocrinology. 2006 Jun;147(6):3027-39. Epub 2006 Feb 23. |
| Immunogen | Ni-NTA purified truncated recombinant GSK3 alpha expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIFWB |
| Western Blot:1:500 - 1:2,000 IF(1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GSK3A |
|---|---|
| Alternative Names | GSK-3 alpha |
Application
Immunofluorescense analysis hof Hela cells using GSK3a antibody showing cytoplasmic localizaiton.
Application
Western blot analysis using GSK3 monoclonal antibody against truncated GSK3alpha recombinant protein| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IF(1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | GSK3 alpha monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
