Application
Immunohistochemical analysis of paraffin - embedded human prostate tissue showing cytoplasmic location using GSTP1 antibody with DAB staining.Application
Western blot analysis using GSTP1 monoclonal antibody against full - length GSTP1 recombinant protein.| Product Name | GSTP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FAEES3, GST3, GST class-piPI, DFN7, GSTP, FAEES3, HEL-S-22 |
| Product description | GSTP1 (glutathione-S-transferase, pi 1), also called GST3/DFN7, which is located on chromosome 11q13 , is a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 act like a tumor suppressor gene, which when inactivated leads to tumor growth, and the -class glutathione S-transferase is commonly inactivated by somatic CpGisland hypermethylation in cancers of the prostate, liver, and breast. Methylation of regulatory sequences at the GSTP1 gene locus is found in the vast majority (>90%) of prostate carcinomas and is associated with transcriptional down-regulation.1) Kimihiko Satoh, Ken Itoh, Masayuki Yamamoto. 2002. Carcinogenesis. 23: 457 - 462. |
| Immunogen | Ni-NTA purified truncated recombinant GSTP1 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GSTP1 |
|---|---|
| Alternative Names | FAEES3 GST3 GST class-piPI DFN7 GSTP FAEES3 HEL-S-22 |
Application
Immunohistochemical analysis of paraffin - embedded human prostate tissue showing cytoplasmic location using GSTP1 antibody with DAB staining.
Application
Western blot analysis using GSTP1 monoclonal antibody against full - length GSTP1 recombinant protein.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | GSTP1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
