GTF2E2 alpha's interaction with RNA polymerase II is subsequent to the entry of RNA polymerase II into the transcription cycle1) Watanabe, T., et al., (2003) Mol. Cell. Biol. 23 (8), 2914-2926.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GTF2E2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
GTF2E2 antibody can be used for detection of GTF2E2 by ELISA at 1:312500. GTF2E2 antibody can be used for detection of GTF2E2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
GTF2E2 antibody can be used for detection of GTF2E2 by ELISA at 1:312500. GTF2E2 antibody can be used for detection of GTF2E2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store GTF2E2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.