Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
Western blot analysis of GTF2E2 using rabbit polyclonal GTF2E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.| Product Name | GTF2E2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription initiation factor IIE subunit beta, TFIIE-beta, General transcription factor IIE subunit 2, GTF2E2, TF2E2 |
| Product description | GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.1) Cirulli, E.T., et al. Eur. J. Hum. Genet. 18(7):815-820(2010) |
| Immunogen | This GTF2E2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the C-terminal region of human GTF2E2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GTF2E2 |
|---|---|
| Alternative Names | Transcription initiation factor IIE subunit beta TFIIE-beta General transcription factor IIE subunit 2 GTF2E2 TF2E2 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
Western blot analysis of GTF2E2 using rabbit polyclonal GTF2E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GTF2E2 gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
